Composite

Part:BBa_K2224004:Design

Designed by: Yunchang Zhang   Group: iGEM17_SMS_Shenzhen   (2017-10-24)


SacB promoter+RBS+SRRz+Terminater


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was taken as a way of for our E. coli to conduct cell lysis. However, after we realizing that the SacB promoter did not have the ability to sense sucrose and trigger expression pathway specifically, we dropped this system.


Source

SacB promotor is from the genomic sequence of Bacillus subtilis subsp. subtilis str. 168. SRRz is from the genomic sequence of Bacteriophage 21.

References